BTN2A1 Protein (AA 29-248) (His tag,DYKDDDDK Tag)

Catalog No. ABIN7504248

This Recombinant BTN2A1 protein is produced in HEK-293 Cells.

Quick Overview for BTN2A1 Protein (AA 29-248) (His tag,DYKDDDDK Tag) (ABIN7504248)

Target: BTN2A1 (butyrophilin, Subfamily 2, Member A1 (BTN2A1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-248

Purification tag / Conjugate: This BTN2A1 protein is labelled with His tag,DYKDDDDK Tag.

Purpose: Human BTN2A1&BTN3A1 complex Protein

Sequence: Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail

Specificity: Uni-Prot: Q7KYR7-1 (BTN2A1), O00481-1 (BTN3A1)

Characteristics: Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag and Flag tag at the C-terminus.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BTN2A1 (butyrophilin, Subfamily 2, Member A1 (BTN2A1))

Alternative Name: BTN2A1

Background: BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.

Molecular Weight: 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa (BTN2A1) and 33-38 kDa (BTN3A1) based on Tris-Bis PAGE result.

Pathways: Activated T Cell Proliferation