BTN2A1 Protein (AA 29-248) (His tag,DYKDDDDK Tag)
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- Target See all BTN2A1 Proteins
- BTN2A1 (butyrophilin, Subfamily 2, Member A1 (BTN2A1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-248
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BTN2A1 protein is labelled with His tag,DYKDDDDK Tag.
- Purpose
- Human BTN2A1&BTN3A1 complex Protein
- Sequence
- Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail
- Specificity
- Uni-Prot: Q7KYR7-1 (BTN2A1), O00481-1 (BTN3A1)
- Characteristics
- Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag and Flag tag at the C-terminus.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product BTN2A1 Protein
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butyrophilin, Subfamily 2, Member A1 (BTN2A1) (AA 29-248) protein (His tag)
BTN2A1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
butyrophilin, Subfamily 2, Member A1 (BTN2A1) (AA 1-334) protein (GST tag)BTN2A1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
butyrophilin, Subfamily 2, Member A1 (BTN2A1) (AA 29-248) protein (His tag)BTN2A1 Origin: Human Host: Yeast Recombinant > 90 % SDS
butyrophilin, Subfamily 2, Member A1 (BTN2A1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)BTN2A1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- BTN2A1 (butyrophilin, Subfamily 2, Member A1 (BTN2A1))
- Alternative Name
- BTN2A1 (BTN2A1 Products)
- Background
- BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.
- Molecular Weight
- 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa (BTN2A1) and 33-38 kDa (BTN3A1) based on Tris-Bis PAGE result.
- Pathways
- Activated T Cell Proliferation
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