HLA-A*24:02 (AA 25-305), (Monomer) protein (Biotin,His-Avi Tag,no Peptide)

Catalog No. ABIN7504412

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for HLA-A*24:02 (AA 25-305), (Monomer) protein (Biotin,His-Avi Tag,no Peptide) (ABIN7504412)

Target: HLA-A*24:02

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-305, Monomer

Purification tag / Conjugate: Biotin,His-Avi Tag,no Peptide

Purpose: Biotinylated Human No Peptide HLA-A*24:02&B2M Monomer Protein

Sequence: Gly25-Thr305(HLA-A*24:02) & Ile21-Met119(B2M)

Specificity: Uni-Prot: AAA59600.1 (HLA-A*24:02), P61769 (B2M)

Characteristics: Recombinant Biotinylated Human No Peptide HLA-A*24:02&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*24:02) and Ile21-Met119(B2M).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Application Notes: Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening

Comment:

The HLA-G&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-G. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLA-A*24:02

Background: No Peptide HLA-A*24:02&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*24:02. No Peptide MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Molecular Weight: 48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.