ITCH Protein (AA 485-862)

Catalog No. ABIN7504453

This Recombinant ITCH protein is expressed in Escherichia coli (E. coli).

Quick Overview for ITCH Protein (AA 485-862) (ABIN7504453)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 485-862

Purpose: Human ITCH Protein

Sequence: Arg485-Glu862

Characteristics: Recombinant Human ITCH Protein is expressed from E.coli without tag.It contains Arg485-Glu862.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH

Background: Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation.

Molecular Weight: 44.98 kDa same as Tris-Bis PAGE result.

NCBI Accession: NP_113671

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events