OGN Protein (AA 21-298) (His tag)

Catalog No. ABIN7504488

This Recombinant OGN protein is produced in HEK-293 Cells.

Quick Overview for OGN Protein (AA 21-298) (His tag) (ABIN7504488)

Target: OGN (Osteoglycin (OGN))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 21-298

Purification tag / Conjugate: This OGN protein is labelled with His tag.

Purpose: Human OGN/Osteoglycin Protein

Sequence: Pro21-Phe298

Characteristics: Recombinant Human OGN/Osteoglycin Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro21-Phe298.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: OGN (Osteoglycin (OGN))

Alternative Name: OGN

Background: Osteoglycin (OGN, a.k.a. mimecan) belongs to cluster III of the small leucine-rich proteoglycans (SLRP) of the extracellular matrix (ECM). In vertebrates OGN is a characteristic ECM protein of bone. The function of OGN has mainly been studied in mammals in which it regulates collagen fibrillogenesis, the efficiency of which is increased when it is processed by BMP-1/Tolloid-like metalloproteinases. OGN has a role in wound healing in the cornea, in atherosclerotic lesions and modulates myocardial integrity and remodelling. In addition, OGN enhances the neurite outgrowth promoted by insulin-like growth factor-2 and IGF binding protein-2.

Molecular Weight: 32.83 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

UniProt: P20774

Pathways: Glycosaminoglycan Metabolic Process