PGK1 Protein (AA 1-417) (His tag)

Catalog No. ABIN7504507

This Recombinant PGK1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for PGK1 Protein (AA 1-417) (His tag) (ABIN7504507)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Protein Type: Recombinant

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 1-417

Purification tag / Conjugate: This PGK1 protein is labelled with His tag.

Purpose: Mouse PGK1 Protein

Sequence: Met1-Val417

Characteristics: Recombinant Mouse PGK1 Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus.It contains Met1-Val417.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris, 500 mM NaCl ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: PGK1

Background: Phosphoglycerate kinase 1 (PGK1) is the first critical enzyme to produce ATP in the glycolytic pathway. PGK1 is not only a metabolic enzyme but also a protein kinase, which mediates the tumor growth, migration and invasion through phosphorylation some important substrates.

Molecular Weight: 46.07 kDa same as Tris-Bis PAGE result.

UniProt: P09411

Pathways: Cellular Glucan Metabolic Process