Iba1 Protein (AA 2-147) (His tag)

Catalog No. ABIN7504920

Recombinant Iba1 protein expressed in Escherichia coli (E. coli).

Quick Overview for Iba1 Protein (AA 2-147) (His tag) (ABIN7504920)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-147

Purification tag / Conjugate: This Iba1 protein is labelled with His tag.

Sequence: Ser2-Pro147

Characteristics: Recombinant Human Allograft Inflammatory Factor 1 is produced by our E.coli expression system and the target gene encoding Ser2-Pro147 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: <1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background:

Abbreviation: AIF1

Target Synonym: Allograft Inflammatory Factor 1,AIF-1,Ionized Calcium-Binding Adapter Molecule 1,Protein G1,AIF1,G1,IBA1

Background: Allograft Inflammatory Factor 1 (AIF1) contains two EF-hand domains and exists as a homodimer. AIF1 can be detected in T-lymphocytes and peripheral blood mononuclear cells. AIF1 functions as actin-binding protein that enhances membrane ruffling and RAC activation and can enhance the actin-bundling activity of LCP1. In addition, AIF1 plays a role in RAC signaling and in phagocytosis and may also in macrophage activation and function. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes and plays a role in vascular inflammation.

Molecular Weight:

Calculated MW: 17.7 kDa

Observed MW: 16 kDa

UniProt: P55008

Pathways: Smooth Muscle Cell Migration