APOH Protein (AA 263-345) (His tag)

Catalog No. ABIN7505114

This Recombinant APOH protein is expressed in Escherichia coli (E. coli).

Quick Overview for APOH Protein (AA 263-345) (His tag) (ABIN7505114)

Target: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 263-345

Purification tag / Conjugate: This APOH protein is labelled with His tag.

Sequence: Ser 263-Cys 345

Characteristics: A DNA sequence encoding the Human APOH protein (P02749) (Ser 263-Cys 345) was expressed with a N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Alternative Name: APOH

Background:

Abbreviation: APOH

Target Synonym: Beta-2-Glycoprotein 1,APC inhibitor,Apolipoprotein H,Apo-H,Beta-2-Glycoprotein I,B2GPIBeta(2)GPI,APOH,B2G1,B2GP1,BG

Background: Apolipoprotein H (APOH), also known as Beta-2-glycoprotein 1, Activated protein C-binding protein, B2GPI, and B2G1, is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. It is an essential cofactor for the binding of certain antiphospholipid antibodies (APA) to anionic phospholipid. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. APOH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. The activity of APOH appears to involve the binding of agglutenating, negatively charged compounds, and inhibits agglutenation by the contact activation of the intrinsic blood coagulation pathway. APOH causes a reduction of the prothrombinase binding sites on platelets and reduces the activation caused by collagen when thrombin is present at physiological serum concentrations of APOH suggesting a regulatory role of APOH in coagulation. APOH plasma concentrations are strongly associated to metabolic syndrome alterations and vascular disease in type 2 diabetic and could be considered as a clinical marker of cardiovascular risk. APOH is found on several classes of lipoproteins, and is involved in the activation of lipoprotein lipase in lipid metabolism. This single-chain glycoprotein also has been implicated in several physiologic pathways including coagulation and the production of hypertension, which are related to the pathogenesis of primary cerebral hemorrhage (PICH).

Molecular Weight:

Calculated MW: 9.02 kDa

Observed MW: 12 kDa

UniProt: P02749