PCNA Protein (AA 1-261) (His tag)

Catalog No. ABIN7505183

Product Details

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Protein Type: Recombinant

Protein Characteristics: AA 1-261

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PCNA protein is labelled with His tag.

Sequence: Met 1-Ser 261

Characteristics: A DNA sequence encoding the Human PCNA protein (P12004 ) (Met 1-Ser 261) was expressed with N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA (PCNA Products)

Background:

Abbreviation: PCNA

Target Synonym: ATLD2,cb16,Cyclin,DNA polymerase delta auxiliary protein,Mutagen-sensitive 209 protein,Polymerase delta accessory protein,Proliferating cell nuclear antigen

Background: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Molecular Weight:

Calculated MW: 28.6 kDa

Observed MW: 38 kDa

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy