PCNA Protein (His tag)

Catalog No. ABIN7317410

Product Details

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This PCNA protein is labelled with His tag.

Purpose: Recombinant Human PCNA Protein (His Tag)

Sequence: Met 1-Ser 261

Characteristics: A DNA sequence encoding the mature form of human PCNA (P12004) (Met 1-Ser 261) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Na3PO4, 300 mM NaCl, 10 % glycerol, pH 7.0, 2 mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA (PCNA Products)

Synonyms: PCNAR Protein, Pcna/cyclin Protein, pcna Protein, AO090003000846 Protein, 53/13 Protein, CG9193 Protein, DmPCNA Protein, Dmel\\CG9193 Protein, MUS209 Protein, Mus209 Protein, PCNA Protein, PCNA/mus209 Protein, Pcna Protein, dPCNA Protein, l(2)02448 Protein, l(2)56F Protein, l(2)56Fa Protein, l(2)s1534 Protein, cb16 Protein, fb36g03 Protein, wu:fa28e03 Protein, wu:fb36g03 Protein, pcna-A Protein, proliferating cell nuclear antigen Protein, DNA polymerase III sliding clamp Protein, Proliferating cell nuclear antigen Protein, Proliferating cell nuclear antigen-like Protein, proliferating cell nuclear antigen L homeolog Protein, proliferating cell nuclear antigen 1 Protein, PCNA Protein, Pcna Protein, pcna Protein, MMP_RS08810 Protein, ANI_1_512014 Protein, AOR_1_1500154 Protein, MGC53867 Protein, pcna.L Protein, pcna1 Protein, LOC106420145 Protein, pcn1 Protein

Background:

Background: Proliferating Cell Nuclear Antigen (PCNA) is a protein only expresse in nomal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.

Synonym: ATLD2

Molecular Weight: 31 kDa

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy