RBBP6 Protein (AA 1-313) (His tag)

Catalog No. ABIN7505272

This Recombinant RBBP6 protein is produced in Escherichia coli (E. coli).

Quick Overview for RBBP6 Protein (AA 1-313) (His tag) (ABIN7505272)

Target: RBBP6 (Retinoblastoma Binding Protein 6 (RBBP6))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-313

Purification tag / Conjugate: This RBBP6 protein is labelled with His tag.

Sequence: Met 1-Lys 313

Characteristics: A DNA sequence encoding the Human PACT protein (O75569) (Met 1-Lys 313) was expressed with a N-His&C-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: RBBP6 (Retinoblastoma Binding Protein 6 (RBBP6))

Alternative Name: PACT

Background:

Abbreviation: PACT

Target Synonym: Interferon-inducible double-stranded RNA-dependent protein kinase activator A,PRKRA,PKR-associated protein X

Background: Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.

Molecular Weight:

Calculated MW: 34.32 kDa

Observed MW: 38 kDa

UniProt: O75569

Pathways: Regulatory RNA Pathways