Fatty Acid Synthase Protein (FASN) (AA 2201-2511) (His tag)

Catalog No. ABIN7505310

Recombinant Fatty Acid Synthase protein expressed in Escherichia coli (E. coli).

Quick Overview for Fatty Acid Synthase Protein (FASN) (AA 2201-2511) (His tag) (ABIN7505310)

Target: Fatty Acid Synthase (FASN)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2201-2511

Purification tag / Conjugate: This Fatty Acid Synthase protein is labelled with His tag.

Sequence: Ala 2201-Gly 2511

Characteristics: A DNA sequence encoding the Human FattyAcidSynthase protein (P49327) (Ala 2201-Gly 2511) was expressed with a C-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Fatty Acid Synthase (FASN)

Alternative Name: FattyAcidSynthase

Background:

Abbreviation: FattyAcidSynthase

Target Synonym: Fatty acid synthase,FASN,Type I fatty acid synthase,

Background: FASN (Fatty Acid Synthase, also FAS) is a cytosolic 270-280 kDa member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes, and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA, and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length ands contains multiple domains, including a beta -ketoacyl synthase domain (aa 1-414), an acyl and malonyl transferase domain (aa 429-817), an enoyl reductase domain (aa 1563-1863), a beta -ketoacyl reductase domain (aa 1864-2118) and a thioesterase domain (aa 2207?2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 9-212, human FASN shares 92 % aa identity with mouse FASN.

Molecular Weight:

Calculated MW: 34.1 kDa

Observed MW: 35 kDa

UniProt: P49327

Pathways: AMPK Signaling