Osteopontin Protein (AA 17-303) (His tag)

Catalog No. ABIN7505465

This Recombinant Osteopontin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Osteopontin Protein (AA 17-303) (His tag) (ABIN7505465)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Protein Type: Recombinant

Origin: Pig

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 17-303

Purification tag / Conjugate: This Osteopontin protein is labelled with His tag.

Sequence: Leu 17-Asn 303

Characteristics: A DNA sequence encoding the Porcine OPN/Osteopontin protein (P14287) (Leu 17-Asn 303) was expressed with N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: OPN

Background:

Abbreviation: Porcine OPN,Osteopontin

Target Synonym: BNSP,Bone sialoprotein 1,BSP I,ETA 1,Nephropontin,Secreted phosphoprotein 1,SPP 1Urinary stone protein,Uroponti

Background: Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80 % amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage.

Molecular Weight:

Calculated MW: 31.46 kDa

Observed MW: 24 kDa

UniProt: P14287

Pathways: Regulation of Cell Size