Adrenomedullin Protein (ADM) (AA 21-185) (His tag)

Catalog No. ABIN7505525

This Recombinant Adrenomedullin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Adrenomedullin Protein (ADM) (AA 21-185) (His tag) (ABIN7505525)

Target: Adrenomedullin (ADM)

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-185

Purification tag / Conjugate: This Adrenomedullin protein is labelled with His tag.

Sequence: Thr 21-Leu 185

Characteristics: A DNA sequence encoding the Rat Adrenomedullin/ADM protein (P43145) (Thr 21-Leu 185) was expressed with a N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Adrenomedullin (ADM)

Alternative Name: Adrenomedullin

Target Type: Hormone

Background:

Abbreviation: Adrenomedullin,ADM

Target Synonym: ADM precursor,Adrenomedullin,PAMP,Preproadrenomedullin,ProAM N terminal 20 peptide,ProAM N20

Background: Adrenomedullin (ADM, also AM) is a secreted, monomeric, 6 kDa member of the Adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. ADM has multiple functions, including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The ADM preproprecursor is 185 amino acids (aa) in length. It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-91), the ADM precursor (amidation is required for maturation) (aa 94 143), and a C-terminal propeptide (aa 150-185). The ADM precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated ADM (aa 94 143).

Molecular Weight:

Calculated MW: 18.04 kDa

Observed MW: 25 kDa

UniProt: P43145

Pathways: Hormone Transport, Hormone Activity, C21-Steroid Hormone Metabolic Process, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Tube Formation