IgG1 Fc (AA 99-330) protein (Fc Tag)

Catalog No. ABIN7505599

This Recombinant protein is produced in HEK-293 Cells.

Quick Overview for IgG1 Fc (AA 99-330) protein (Fc Tag) (ABIN7505599)

Target: IgG1 Fc

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 99-330

Purification tag / Conjugate: Fc Tag

Sequence: Glu99-Lys330

Characteristics: A DNA sequence encoding theHuman IgG1 Fc protein (P01857) (Glu99-Lys330) was expressed with a C-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IgG1 Fc

Background:

Abbreviation: IgG1 Fc

Target Synonym: Ig gamma-1 chain C region,Ig gamma-1 chain C region EU,Ig gamma-1 chain C region KOL,Ig gamma-1 chain C region NIE

Background: Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens.The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

Molecular Weight:

Calculated MW: 40 kDa

Observed MW: 35 kDa

UniProt: P01857