PVRL1 Protein (AA 1-334) (His tag)

Catalog No. ABIN7505696

Product Details

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Protein Type: Recombinant

Protein Characteristics: AA 1-334

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PVRL1 protein is labelled with His tag.

Sequence: Met1-Thr334

Characteristics: A DNA sequence encoding the Human PVRL1/NECTIN1/CD111 protein (Q15223) (Met1-Thr334) was expressed with a C-His.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Alternative Name: PVRL1 (PVRL1 Products)

Background:

Abbreviation: PVRL1,NECTIN1,CD111

Target Synonym: Poliovirus Receptor-Related Protein 1,Herpes Virus Entry Mediator C,Herpesvirus Entry Mediator C,HveC,Herpesvirus Ig-Like Receptor,HIgR,Nectin-1,CD111,PVRL1,HVEC,PRR1,ED4,HIgR,HV1S,HVEC,nectin-1,OFC7,PRR,PVRR,PVRR1,SK-12

Background: Nectin-1 is a type I transmembrane glycoprotein belonging to the Ig superfamily. Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Nectin ECDs contain three Ig like domains: an N terminal V type that mediates ligand binding, and two C2 type. Nectin-1 binds viral Glycoprotein D to mediate Herpesvirus (but not Poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts. In forming adherens junctions and synapses, Nectin-1 and Nectin-3 initiate cell-cell interactions, recruiting αvβ3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins. These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 and Nectin-3 have been found to localize assymetrically along the chemical synapse, with Nectin-1 primarily on the axonal side and Nectin-3 on the dendritic side. Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia. Nectin-1 downregulation in epithelial cancers is mediated in part by ectodomain shedding, but it may contribute to invasiveness.

Molecular Weight:

Calculated MW: 36.63 kDa

Observed MW: 45 kDa

UniProt: Q15223

Pathways: Cell-Cell Junction Organization