SERPINC1 Protein (AA 1-465) (His tag)

Catalog No. ABIN7505777

This Recombinant SERPINC1 protein is expressed in HEK-293 Cells.

Quick Overview for SERPINC1 Protein (AA 1-465) (His tag) (ABIN7505777)

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-465

Purification tag / Conjugate: This SERPINC1 protein is labelled with His tag.

Sequence: Met 1-Asn 465

Characteristics: A DNA sequence encoding the Rat antithrombin-III protein (Q5M7T5) (Met1-Asn465) was expressed with a C-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Alternative Name: Antithrombin-III

Background:

Abbreviation: Antithrombin-III,Serpinc1

Target Synonym: AT3 Protein,AT3D,ATIII Protein,Serpin C1 Protein,SerpinC1

Background: SerpinC1, also known as antithrombin III (AT III), is a member of the serpin superfamily of serine protease inhibitors, and has been found to be a marker for disseminated intravascular coagulation (DIC) and to be of prognostic significance in septic patients. SerpinC1 synthesized in the liver is the principal plasma serpin of blood coagulation proteases and inhibits thrombin and other factors such as Xa by the formation of covalently linked complexes. Thus it is one of the most important coagulation inhibitors and the fundamental enzyme for the therapeutical action of heparin. In common with SerpinA5 and D1, the inhibitory activity of SerpinC1 undergoes a dramatic increase in the presence of heparin and other glycosaminoglycans. ATIII mediates the promotion of prostaglandin release, an inhibitor of leucocyte activation and downregulator of many proinflammatory cytokines. Antithrombin III exerts anti-inflammatory properties in addition to its anti-coagulative mechanisms. In animal models of sepsis, ATIII affected cytokine plasma concentrations with a decrease of pro-inflammatory cytokines. The deficiency or functional abnormality of ATIII may result in an increased risk of thromboembolic disease, such as deep vein thrombosis and pulmonary embolism. In addition, it has been reported that SerpinC1 can alter or influence inflammatory processes via inhibition of NF-κB activation or actin polymerization.

Molecular Weight:

Calculated MW: 51.04 kDa

Observed MW: 60 kDa

UniProt: Q5M7T5