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Cor a 1 (Corylus Avellana) Protein

Recombinant protein expressed in Human.

Analysis of presence of sIgE antibodies specific to Cor a 1 (a common hazel antigen) in plasma of a patient with allergic reaction to common birch pollen (confirmed by routine clinical test). (Cor a 1 (Corylus Avellana) Protein)

Recombinant allergen rCor a 1 purity verification. (Cor a 1 (Corylus Avellana) Protein)

ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen rCor a 1 and allergen-specific human plasma IgG4 antibodies of Corylus avellana positive donor. (Cor a 1 (Corylus Avellana) Protein)

Flow cytometry dot-plot staining pattern of rCor a 1 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies . (Cor a 1 (Corylus Avellana) Protein)

Reactivity of rCor a 1 with sIgE antibodies in plasma of a patient with confirmed presence (routine clinical test) of specific IgE antibodies to common hazel pollen. (Cor a 1 (Corylus Avellana) Protein)

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Catalog No. ABIN7506045

Quick Overview for Cor a 1 (Corylus Avellana) Protein (ABIN7506045)

Target

Cor a 1 (Corylus Avellana)

Protein Type

Recombinant

Origin

Hazel

Source

Human

Application

ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Purpose

rCor a 1 (Corylus avellana 1.0401)

Characteristics

Recombinant protein rCor a 1 is expressed in Escherichia coli. DNA sequence encoding 174 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,1 kDa.

Purification

Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
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Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Lyophilized

Preservative

Azide free

Storage

-20 °C/-80 °C

Storage Comment

Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Target

Cor a 1 (Corylus Avellana)

Target Type

Bacteria Protein

UniProt

Q9SWR4

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