MMP13 Protein (His tag)

Catalog No. ABIN7534286

This Recombinant MMP13 protein is expressed in HEK-293 Cells.

Quick Overview for MMP13 Protein (His tag) (ABIN7534286)

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This MMP13 protein is labelled with His tag.

Purpose: Active Recombinant Human MMP-13 Protein

Sequence: LPLPSGGDED DLSEEDLQFA ERYLRSYYHP TNLAGILKEN AASSMTERLR EMQSFFGLEV TGKLDDNTLD VMKKPRCGVP DVGEYNVFPR TLKWSKMNLT YRIVNYTPDM THSEVEKAFK KAFKVWSDVT PLNFTRLHDG IADIMISFGI KEHGDFYPFD GPSGLLAHAF PPGPNYGGDA HFDDDETWTS SSKGYNLFLV AAHEFGHSLG LDHSKDPGAL MFPIYTYTGK SHFMLPDDDV QGIQSLYGPG DEDPNPKHPK TPDKCDPSLS LDAITSLRGE TMIFKDRFFW RLHPQQVDAE LFLTKSFWPE LPNRIDAAYE HPSHDLIFIF RGRKFWALNG YDILEGYPKK ISELGLPKEV KKISAAVHFE DTGKTLLFSG NQVWRYDDTN HIMDKDYPRL IEEDFPGIGD KVDAVYEKNG YIYFFNGPIQ FEYSIWSNRI VRVMPANSIL WC

Specificity: Leu20-Cys471

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured in a cell migration assay using A549 cells. 10 ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration.2.Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >2900 pmol/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP-13

Background:

Description: Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is proteolytically processed to generate the mature protease. MMP-13 is distinguished from the other human collagenases ,it cleaves type II collagen more efficiently than types I and III. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis.

Name: CLG3, MANDP1, MDST, MMP-13,MMP13,MANDP1,MDST,MMP-13

Gene ID: 4322

UniProt: P45452