NEFL Protein
Quick Overview for NEFL Protein (ABIN7536068)
Target
See all NEFL ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Recombinant Human NF-L/NEFL Protein
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Sequence
- SSGSLMPSLE NLDLSQVAAI SNDLKSIRTQ EKAQLQDLND RFASFIERVH ELEQQNKVLE AELLVLRQKH SEPSRFRALY EQEIRDLRLA AEDATNEKQA LQGEREGLEE TLRNLQARYE EEVLSREDAE GRLMEARKGA DEAALARAEL EKRIDSLMDE ISFLKKVHEE EIAELQAQIQ YAQISVEMDV T
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Specificity
- Ser60-Thr250
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- <0.1EU/μg
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Neurofilament, Light Polypeptide (NEFL) (AA 89-400) protein (His tag)
NEFL Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Neurofilament, Light Polypeptide (NEFL) (AA 9-88) protein (Fc Tag)NEFL Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Neurofilament, Light Polypeptide (NEFL) (AA 1-543) protein (GST tag)NEFL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neurofilament, Light Polypeptide (NEFL) (AA 318-543) protein (His tag)NEFL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Neurofilament, Light Polypeptide (NEFL) (AA 2-543) protein (Fc Tag)NEFL Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- NEFL (Neurofilament, Light Polypeptide (NEFL))
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Alternative Name
- NF-L/NEFL
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Background
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Description: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Name: NEFL,CMT1F,CMT2E,NF-L,NF68,NFL,PPP1R110
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Gene ID
- 4747
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UniProt
- P07196
Target
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