Nuclease Protein

Catalog No. ABIN7520371

This Recombinant Nuclease protein is produced in Yeast (Pichia pastoris).

Quick Overview for Nuclease Protein (ABIN7520371)

Target: Nuclease

Protein Type: Recombinant

Biological Activity: Active

Origin: Serratia marcescens

Source: Yeast (Pichia pastoris)

Purity: > 95 % by SDS-PAGE.

Product Details

Purpose: Active Recombinant Serratia marcescens Nuclease

Sequence: DTLESIDNCA VGCPTGGSSN VSIVRHAYTL NNNSTTKFAN WVAYHITKDT PASGKTRNWK TDPALNPADT LAPADYTGAN AALKVDRGHQ APLASLAGVS DWESLNYLSN ITPQKSDLNQ GAWARLEDQE RKLIDRADIS SVYTVTGPLY ERDMGKLPGT QKAHTIPSAY WKVIFINNSP AVNHYAAFLF DQNTPKGADF CQFRVTVDEI EKRTGLIIWA GLPDDVQASL KSKPGVLPEL MGCKN

Specificity: Asp22-Asn266

Endotoxin Level: <0.1EU/μg

Biological Activity Comment: 1.One unit (U) is defined as the amount of enzyme required to change the absorption value of A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL.|2.Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37°C for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Solution in 50 % glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl₂, and 20 mM NaCl.

Storage: -20 °C

Storage Comment: This product is stable at -15°C ~ -25°C for up to 2 year from the date of receipt.|For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Target Details

Target: Nuclease

Background:

Description: Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.

Name: Endonuclease, Nuclease, nucA, nuc

Gene ID: 66715254

UniProt: P13717