Osteopontin Protein (His tag)
Quick Overview for Osteopontin Protein (His tag) (ABIN7534598)
Target
See all Osteopontin (SPP1) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This Osteopontin protein is labelled with His tag.
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Purpose
- Active Recombinant Human Osteopontin/SPP-1 Protein
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Sequence
- IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQTLPSKSNE SHDHMDDMDD EDDDDHVDSQ DSIDSNDSDD VDDTDDSHQS DESHHSDESD ELVTDFPTDL PATEVFTPVV PTVDTYDGRG DSVVYGLRSK SKKFRRPDIQ YPDATDEDIT SHMESEELNG AYKAIPVAQD LNAPSDWDSR GKDSYETSQL DDQSAETHSH KQSRLYKRKA NDESNEHSDV IDSQELSKVS REFHSHEFHS HEDMLVVDPK SKEEDKHLKF RISHELDSAS SEVN
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Specificity
- Ile17-Asn300
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human SPP1 Protein at 1 μg/mL (100 μL/well) can bind Osteopontin Rabbit mAb with a linear range of 0.976-11.58 ng/mL.
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Secreted phosphoprotein 1 (SPP1) (AA 17-300) protein (His tag)
SPP1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 17-294) protein (His tag)SPP1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 28-61) protein (His-IF2DI Tag)SPP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Secreted phosphoprotein 1 (SPP1) (AA 1-300) protein (GST tag)SPP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Secreted phosphoprotein 1 (SPP1) proteinSPP1 Origin: Human Native > 90 % (SDS-PAGE)
Secreted phosphoprotein 1 (SPP1) (AA 17-300), (partial) protein (His tag)SPP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Secreted phosphoprotein 1 (SPP1) (AA 17-287) (Active) protein (His tag)SPP1 Origin: Human Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
Secreted phosphoprotein 1 (SPP1) (AA 17-317) (Active) protein (His tag)SPP1 Origin: Rat Host: HEK-293 Cells > 95 % AcA, CC Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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Alternative Name
- Osteopontin/SPP-1
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Background
- BNSP,BSPI,ETA-1,OPN,Osteopontin,SPP1
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Gene ID
- 6696
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UniProt
- P10451-5
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Pathways
- Regulation of Cell Size
Target
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