Adhesion G Protein-Coupled Receptor G1 (ADGRG1) Protein

Catalog No. ABIN7538124

This Synthetic Nanodisc protein is expressed in Mammalian Cells.

Quick Overview for Adhesion G Protein-Coupled Receptor G1 (ADGRG1) Protein (ABIN7538124)

Target: Adhesion G Protein-Coupled Receptor G1 (ADGRG1)

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Product Details

Purpose: Human ADGRG1 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Adhesion G Protein-Coupled Receptor G1 (ADGRG1)

Alternative Name: ADGRG1

Background: A member of the G protein-coupled receptor family and regulates brain cortical patterning. The encoded protein binds specifically to transglutaminase 2, a component of tissue and tumor stroma implicated as an inhibitor of tumor progression. Mutations in this gene are associated with a brain malformation known as bilateral frontoparietal polymicrogyria.

Molecular Weight: The human full length ADGRG1 protein has a MW of 77.7 kDa

UniProt: Q9Y653