SPAG4 Protein (AA 1-437) (His tag)

Catalog No. ABIN7546151

This Recombinant SPAG4 protein is expressed in HEK-293 Cells.

Quick Overview for SPAG4 Protein (AA 1-437) (His tag) (ABIN7546151)

Target: SPAG4 (Sperm Associated Antigen 4 (SPAG4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-437

Purification tag / Conjugate: This SPAG4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant SPAG4 Protein expressed in mammalian cells.

Sequence: MRRSSRPGSA SSSRKHTPNF FSENSSMSIT SEDSKGLRSA EPGPGEPEGR RARGPSCGEP ALSAGVPGGT TWAGSSQQKP APRSHNWQTA CGAATVRGGA SEPTGSPVVS EEPLDLLPTL DLRQEMPPPR VFKSFLSLLF QGLSVLLSLA GDVLVSMYRE VCSIRFLFTA VSLLSLFLSA FWLGLLYLVS PLENEPKEML TLSEYHERVR SQGQQLQQLQ AELDKLHKEV STVRAANSER VAKLVFQRLN EDFVRKPDYA LSSVGASIDL QKTSHDYADR NTAYFWNRFS FWNYARPPTV ILEPHVFPGN CWAFEGDQGQ VVIQLPGRVQ LSDITLQHPP PSVEHTGGAN SAPRDFAVFG LQVYDETEVS LGKFTFDVEK SEIQTFHLQN DPPAAFPKVK IQILSNWGHP RFTCLYRVRA HGVRTSEGAE GSAQGPH Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SPAG4 (Sperm Associated Antigen 4 (SPAG4))

Alternative Name: SPAG4

Background: Sperm-associated antigen 4 protein (Outer dense fiber-associated protein SPAG4) (SUN domain-containing protein 4),FUNCTION: Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. {ECO:0000250|UniProtKB:O55034, ECO:0000250|UniProtKB:Q9JJF2}.

Molecular Weight: 48.2 kDa

UniProt: Q9NPE6

AlphaFold: Q9NPE6