DDI1 Protein (AA 1-396) (His tag)

Catalog No. ABIN7547014

Product Details

Target: DDI1 (DNA-Damage Inducible 1 Homolog 1 (DDI1))

Protein Type: Recombinant

Protein Characteristics: AA 1-396

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This DDI1 protein is labelled with His tag.

Purpose: Made-to-order recombinant DDI1 Protein expressed in mammalian cells.

Sequence: MLITVYCVRR DLSEVTFSLQ VSPDFELRNF KVLCEAESRV PVEEIQIIHM ERLLIEDHCS LGSYGLKDGD IVVLLQKDNV GPRAPGRAPN QPRVDFSGIA VPGTSSSRPQ HPGQQQQRTP AAQRSQGLAS GEKVAGLQGL GSPALIRSML LSNPHDLSLL KERNPPLAEA LLSGSLETFS QVLMEQQREK ALREQERLRL YTADPLDREA QAKIEEEIRQ QNIEENMNIA IEEAPESFGQ VTMLYINCKV NGHPLKAFVD SGAQMTIMSQ ACAERCNIMR LVDRRWAGVA KGVGTQRIIG RVHLAQIQIE GDFLQCSFSI LEDQPMDMLL GLDMLRRHQC SIDLKKNVLV IGTTGTQTYF LPEGELPLCS RMVSGQDESS DKEITHSVMD SGRKEH Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DDI1 (DNA-Damage Inducible 1 Homolog 1 (DDI1))

Alternative Name: DDI1 (DDI1 Products)

Background: Protein DDI1 homolog 1 (EC 3.4.23.-),FUNCTION: Probable aspartic protease (Probable). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI2, for cellular survival following replication stress. Together or redudantly with DDI2, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed:29290612). {ECO:0000269|PubMed:29290612, ECO:0000305|PubMed:21266539, ECO:0000305|PubMed:29290612}.

Molecular Weight: 44.1 kDa

UniProt: Q8WTU0

AlphaFold: Q8WTU0