DDX18 Protein (AA 1-670) (His tag)

Catalog No. ABIN7547034

Recombinant DDX18 protein expressed in HEK-293 Cells.

Quick Overview for DDX18 Protein (AA 1-670) (His tag) (ABIN7547034)

Target: DDX18 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 18 (DDX18))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-670

Purification tag / Conjugate: This DDX18 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant DDX18 Protein expressed in mammalian cells.

Sequence: MSHLPMKLLR KKIEKRNLKL RQRNLKFQGA SNLTLSETQN GDVSEETMGS RKVKKSKQKP MNVGLSETQN GGMSQEAVGN IKVTKSPQKS TVLTNGEAAM QSSNSESKKK KKKKRKMVND AEPDTKKAKT ENKGKSEEES AETTKETENN VEKPDNDEDE SEVPSLPLGL TGAFEDTSFA SLCNLVNENT LKAIKEMGFT NMTEIQHKSI RPLLEGRDLL AAAKTGSGKT LAFLIPAVEL IVKLRFMPRN GTGVLILSPT RELAMQTFGV LKELMTHHVH TYGLIMGGSN RSAEAQKLGN GINIIVATPG RLLDHMQNTP GFMYKNLQCL VIDEADRILD VGFEEELKQI IKLLPTRRQT MLFSATQTRK VEDLARISLK KEPLYVGVDD DKANATVDGL EQGYVVCPSE KRFLLLFTFL KKNRKKKLMV FFSSCMSVKY HYELLNYIDL PVLAIHGKQK QNKRTTTFFQ FCNADSGTLL CTDVAARGLD IPEVDWIVQY DPPDDPKEYI HRVGRTARGL NGRGHALLIL RPEELGFLRY LKQSKVPLSE FDFSWSKISD IQSQLEKLIE KNYFLHKSAQ EAYKSYIRAY DSHSLKQIFN VNNLNLPQVA LSFGFKVPPF VDLNVNSNEG KQKKRGGGGG FGYQKTKKVE KSKIFKHISK KSSDSRQFSH Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DDX18 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 18 (DDX18))

Alternative Name: DDX18

Background: ATP-dependent RNA helicase DDX18 (EC 3.6.4.13) (DEAD box protein 18) (Myc-regulated DEAD box protein) (MrDb),FUNCTION: Probable RNA-dependent helicase.

Molecular Weight: 75.4 kDa

UniProt: Q9NVP1

AlphaFold: Q9NVP1