ELF1 Protein (AA 1-619) (His tag)
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- Target See all ELF1 Proteins
- ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-619
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ELF1 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant ELF1 Protein expressed in mammalian cells.
- Sequence
- MAAVVQQNDL VFEFASNVME DERQLGDPAI FPAVIVEHVP GADILNSYAG LACVEEPNDM ITESSLDVAE EEIIDDDDDD ITLTVEASCH DGDETIETIE AAEALLNMDS PGPMLDEKRI NNNIFSSPED DMVVAPVTHV SVTLDGIPEV METQQVQEKY ADSPGASSPE QPKRKKGRKT KPPRPDSPAT TPNISVKKKN KDGKGNTIYL WEFLLALLQD KATCPKYIKW TQREKGIFKL VDSKAVSRLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK EMPKDLIYIN DEDPSSSIES SDPSLSSSAT SNRNQTSRSR VSSSPGVKGG ATTVLKPGNS KAAKPKDPVE VAQPSEVLRT VQPTQSPYPT QLFRTVHVVQ PVQAVPEGEA ARTSTMQDET LNSSVQSIRT IQAPTQVPVV VSPRNQQLHT VTLQTVPLTT VIASTDPSAG TGSQKFILQA IPSSQPMTVL KENVMLQSQK AGSPPSIVLG PAQVQQVLTS NVQTICNGTV SVASSPSFSA TAPVVTFSPR SSQLVAHPPG TVITSVIKTQ ETKTLTQEVE KKESEDHLKE NTEKTEQQPQ PYVMVVSSSN GFTSQVAMKQ NELLEPNSF Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-612) protein (His tag)
custom-made ELF1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 366-412) protein (His tag)ELF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-619) protein (GST tag)ELF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
- Alternative Name
- ELF1 (ELF1 Products)
- Background
- ETS-related transcription factor Elf-1 (E74-like factor 1),FUNCTION: Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. {ECO:0000269|PubMed:8756667}.
- Molecular Weight
- 67.5 kDa
- UniProt
- P32519
- AlphaFold
- P32519
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