ENY2 Protein (AA 1-101) (His tag)

Catalog No. ABIN7547384

This Recombinant ENY2 protein is expressed in HEK-293 Cells.

Quick Overview for ENY2 Protein (AA 1-101) (His tag) (ABIN7547384)

Target: ENY2 (Enhancer of Yellow 2 Homolog (ENY2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-101

Purification tag / Conjugate: This ENY2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant ENY2 Protein expressed in mammalian cells.

Sequence: MVVSKMNKDA QMRAAINQKL IETGERERLK ELLRAKLIEC GWKDQLKAHC KEVIKEKGLE HVTVDDLVAE ITPKGRALVP DSVKKELLQR IRTFLAQHAS L Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ENY2 (Enhancer of Yellow 2 Homolog (ENY2))

Alternative Name: ENY2

Background: Transcription and mRNA export factor ENY2 (Enhancer of yellow 2 transcription factor homolog),FUNCTION: Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation (PubMed:18206972, PubMed:21746879). As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (PubMed:23591820). {ECO:0000269|PubMed:18206972, ECO:0000269|PubMed:21746879, ECO:0000269|PubMed:23591820}.

Molecular Weight: 11.5 kDa

UniProt: Q9NPA8

AlphaFold: Q9NPA8