EMC9 Protein (AA 1-208) (His tag)

Catalog No. ABIN7547421

Product Details

Target: EMC9 (ER Membrane Protein Complex Subunit 9 (EMC9))

Protein Type: Recombinant

Protein Characteristics: AA 1-208

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This EMC9 protein is labelled with His tag.

Purpose: Made-to-order recombinant EMC9 Protein expressed in mammalian cells.

Sequence: MGEVEISALA YVKMCLHAAR YPHAAVNGLF LAPAPRSGEC LCLTDCVPLF HSHLALSVML EVALNQVDVW GAQAGLVVAG YYHANAAVND QSPGPLALKI AGRIAEFFPD AVLIMLDNQK LVPQPRVPPV IVLENQGLRW VPKDKNLVMW RDWEESRQMV GALLEDRAHQ HLVDFDCHLD DIRQDWTNQR LNTQITQWVG PTNGNGNA Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: EMC9 (ER Membrane Protein Complex Subunit 9 (EMC9))

Alternative Name: EMC9 (EMC9 Products)

Background: ER membrane protein complex subunit 9 (Protein FAM158A),FUNCTION: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). {ECO:0000269|PubMed:29242231, ECO:0000269|PubMed:29809151, ECO:0000269|PubMed:30415835, ECO:0000269|PubMed:32459176, ECO:0000305}.

Molecular Weight: 23.1 kDa

UniProt: Q9Y3B6

AlphaFold: Q9Y3B6