FBXL13 Protein (AA 1-735) (His tag)

Catalog No. ABIN7547769

Product Details

Target: FBXL13 (F-Box and Leucine-Rich Repeat Protein 13 (FBXL13))

Protein Type: Recombinant

Protein Characteristics: AA 1-735

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FBXL13 protein is labelled with His tag.

Purpose: Made-to-order recombinant FBXL13 Protein expressed in mammalian cells.

Sequence: MTPELMIKAC SFYTGHLVKT HFCTWRDIAR TNENVVLAEK MNRAVTCYNF RLQKSVFHHW HSYMEDQKEK LKNILLRIQQ IIYCHKLTII LTKWRNTARH KSKKKEDELI LKHELQLKKW KNRLILKRAA AEESNFPERS SSEVFLVDET LKCDISLLPE RAILQIFFYL SLKDVIICGQ VNHAWMLMTQ LNSLWNAIDF SSVKNVIPDK YIVSTLQRWR LNVLRLNFRG CLLRPKTFRS VSHCRNLQEL NVSDCPTFTD ESMRHISEGC PGVLCLNLSN TTITNRTMRL LPRHFHNLQN LSLAYCRRFT DKGLQYLNLG NGCHKLIYLD LSGCTQISVQ GFRYIANSCT GIMHLTINDM PTLTDNCVKA LVEKCSRITS LVFTGAPHIS DCTFRALSAC KLRKIRFEGN KRVTDASFKF IDKNYPNLSH IYMADCKGIT DSSLRSLSPL KQLTVLNLAN CVRIGDMGLK QFLDGPASMR IRELNLSNCV RLSDASVMKL SERCPNLNYL SLRNCEHLTA QGIGYIVNIF SLVSIDLSGT DISNEGLNVL SRHKKLKELS VSECYRITDD GIQAFCKSSL ILEHLDVSYC SQLSDMIIKA LAIYCINLTS LSIAGCPKIT DSAMEMLSAK CHYLHILDIS GCVLLTDQIL EDLQIGCKQL RILKMQYCTN ISKKAAQRMS SKVQQQEYNT NDPPRWFGYD REGNPVTELD NITSSKGALE LTVKKSTYSS EDQAA Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FBXL13 (F-Box and Leucine-Rich Repeat Protein 13 (FBXL13))

Alternative Name: FBXL13 (FBXL13 Products)

Background: F-box and leucine-rich repeat protein 13 (Dynein regulatory complex subunit 6) (F-box/LRR-repeat protein 13),FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Specifically targets CEP192 isoform 3 for ubiquitin-mediated proteolysis and thereby acts as a regulator of microtubule nucleation activity (PubMed:29348145). {ECO:0000250|UniProtKB:A8JHD7, ECO:0000269|PubMed:29348145}.

Molecular Weight: 83.9 kDa

UniProt: Q8NEE6

AlphaFold: Q8NEE6