FBXL19 Protein (AA 1-694) (His tag)

Catalog No. ABIN7547856

Product Details

Target: FBXL19 (F-Box and Leucine-Rich Repeat Protein 19 (FBXL19))

Protein Type: Recombinant

Protein Characteristics: AA 1-694

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FBXL19 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant FBXL19 Protein expressed in mammalian cells.

Sequence: MGMKVPGKGE SGPSALLTPP MSSSSRGPGA GARRRRTRCR RCRACVRTEC GDCHFCRDMK KFGGPGRMKQ SCLLRQCTAP VLPHTAVCLL CGEAGKEDTV EGEEEKFGLS LMECTICNEI VHPGCLKMGK AEGVINAEIP NCWECPRCTQ EGRTSKDSGE GPGRRRADNG EEGASLGSGW KLTEEPPLPP PPPRRKGPLP AGPPPEDVPG PPKRKEREAG NEPPTPRKKV KGGRERHLKK VGGDACLLRG SDPGGPGLLP PRVLNPSQAF SSCHPGLPPE NWEKPKPPLA SAEGPAVPSP SPQREKLERF KRMCQLLERV PDTSSSSSDS DSDSDSSGTS LSEDEAPGEA RNGRRPARGS SGEKENRGGR RAVRPGSGGP LLSWPLGPAP PPRPPQLERH VVRPPPRSPE PDTLPLAAGS DHPLPRAAWL RVFQHLGPRE LCICMRVCRT WSRWCYDKRL WPRMDLSRRK SLTPPMLSGV VRRQPRALDL SWTGVSKKQL MWLLNRLQGL QELVLSGCSW LSVSALGSAP LPALRLLDLR WIEDVKDSQL RELLLPPPDT KPGQTESRGR LQGVAELRLA GLELTDASLR LLLRHAPQLS ALDLSHCAHV GDPSVHLLTA PTSPLRETLV HLNLAGCHRL TDHCLPLFRR CPRLRRLDLR SCRQLSPEAC ARLAAAGPPG PFRCPEEKLL LKDS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FBXL19 (F-Box and Leucine-Rich Repeat Protein 19 (FBXL19))

Alternative Name: FBXL19 (FBXL19 Products)

Background: F-box/LRR-repeat protein 19 (F-box and leucine-rich repeat protein 19),FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex that plays a role in different processes including cell migration, cell proliferation or cytoskeletal reorganization (PubMed:24684802, PubMed:29522376). Mediates RHOA ubiquitination and degradation in a ERK2-dependent manner (PubMed:23871831). Induces RAC1 and RAC3 degradation by the proteasome system and thereby regulates TGFB1-induced E-cadherin down-regulation and cell migration (PubMed:24684802, PubMed:23512198). Mediates also ubiquitination and degradation of IL-33-induced receptor IL1RL1 and subsequently blocks IL-33-mediated apoptosis (By similarity). Within the nucleus, binds to DNA containing unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed:29276034). Recruits CDK-mediator to chromatin and targets CDK8 to promoters of silent developmental genes leading to induction of these genes during cell differentiation. In addition, plays a critical role in the recruitment of RNF20 to histone H2B leading to H2B mono-ubiquitination (By similarity). {ECO:0000250|UniProtKB:Q6PB97, ECO:0000269|PubMed:23512198, ECO:0000269|PubMed:23871831, ECO:0000269|PubMed:24684802, ECO:0000269|PubMed:29276034, ECO:0000269|PubMed:29522376}.

Molecular Weight: 75.7 kDa

UniProt: Q6PCT2

AlphaFold: Q6PCT2