GPR31 Protein (AA 1-319) (His tag)

Catalog No. ABIN7548084

Recombinant GPR31 protein expressed in HEK-293 Cells.

Quick Overview for GPR31 Protein (AA 1-319) (His tag) (ABIN7548084)

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-319

Purification tag / Conjugate: This GPR31 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant GPR31 Protein expressed in mammalian cells.

Sequence: MPFPNCSAPS TVVATAVGVL LGLECGLGLL GNAVALWTFL FRVRVWKPYA VYLLNLALAD LLLAACLPFL AAFYLSLQAW HLGRVGCWAL HFLLDLSRSV GMAFLAAVAL DRYLRVVHPR LKVNLLSPQA ALGVSGLVWL LMVALTCPGL LISEAAQNST RCHSFYSRAD GSFSIIWQEA LSCLQFVLPF GLIVFCNAGI IRALQKRLRE PEKQPKLQRA QALVTLVVVL FALCFLPCFL ARVLMHIFQN LGSCRALCAV AHTSDVTGSL TYLHSVLNPV VYCFSSPTFR SSYRRVFHTL RGKGQAAEPP DFNPRDSYS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Alternative Name: GPR31

Background: 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor (12-(S)-HETE receptor) (12-HETER) (G-protein coupled receptor 31) (GPR31/12-HETER),FUNCTION: High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE), with much lower affinities for other HETE isomers (PubMed:21712392, PubMed:29227475). 12-S-HETE is a eicosanoid, a 12-lipoxygenase (ALOX12) metabolite of arachidonic acid, involved in many physiologic and pathologic processes (PubMed:26965684, PubMed:28619714, PubMed:29227475). 12-S-HETE-binding leads to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B pathways leading to cell growth (PubMed:21712392, PubMed:29227475). Plays a crucial role for proliferation, survival and macropinocytosis of KRAS-dependent cancer cells by mediating the translocation of KRAS from the endoplasmic reticulum to the plasma membrane (PM) and its association with the PM (PubMed:28619714). Contributes to enhanced immune responses by inducing dendrite protrusion of small intestinal CX3CR1(+) phagocytes for the uptake of luminal antigens (By similarity). Acts also as a key receptor for 12-(S)-HETE-mediated liver ischemia reperfusion injury (PubMed:29227475). {ECO:0000250|UniProtKB:F8VQN3, ECO:0000269|PubMed:21712392, ECO:0000269|PubMed:26965684, ECO:0000269|PubMed:28619714, ECO:0000269|PubMed:29227475}., FUNCTION: Proton-sensing G protein-coupled receptor. {ECO:0000269|PubMed:31119277}.

Molecular Weight: 35.1 kDa

UniProt: O00270

AlphaFold: O00270