RASGRP4 Protein (AA 1-673) (His tag)

Catalog No. ABIN7548102

Recombinant RASGRP4 protein expressed in HEK-293 Cells.

Quick Overview for RASGRP4 Protein (AA 1-673) (His tag) (ABIN7548102)

Target: RASGRP4 (RAS Guanyl Releasing Protein 4 (RASGRP4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-673

Purification tag / Conjugate: This RASGRP4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant RASGRP4 Protein expressed in mammalian cells.

Sequence: MNRKDSKRKS HQECTGKIGG RGRPRQVRRH KTCPSPREIS KVMASMNLGL LSEGGCSEDE LLEKCIQSFD SAGSLCHEDH MLNMVLAMHS WVLPSADLAA RLLTSYQKAT GDTQELRRLQ ICHLVRYWLM RHPEVMHQDP QLEEVIGRFW ATVAREGNSA QRRLGDSSDL LSPGGPGPPL PMSSPGLGKK RKVSLLFDHL ETGELAQHLT YLEFRSFQAI TPQDLRSYVL QGSVRGCPAL EGSVGLSNSV SRWVQVMVLS RPGPLQRAQV LDKFIHVAQR LHQLQNFNTL MAVTGGLCHS AISRLKDSHA HLSPDSTKAL LELTELLASH NNYARYRRTW AGCAGFRLPV LGVHLKDLVS LHEAQPDRLP DGRLHLPKLN NLYLRLQELV ALQGQHPPCS ANEDLLHLLT LSLDLFYTED EIYELSYARE PRCPKSLPPS PFNAPLVVEW APGVTPKPDR VTLGRHVEQL VESVFKNYDP EGRGTISQED FERLSGNFPF ACHGLHPPPR QGRGSFSREE LTGYLLRASA ICSKLGLAFL HTFHEVTFRK PTFCDSCSGF LWGVTKQGYR CRECGLCCHK HCRDQVKVEC KKRPGAKGDA GPPGAPVPST PAPHASCGSE ENHSYTLSLE PETGCQLRHA WTQTESPHPS WETDTVPCPV MDPPSTASSK LDS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RASGRP4 (RAS Guanyl Releasing Protein 4 (RASGRP4))

Alternative Name: RASGRP4

Background: RAS guanyl-releasing protein 4,FUNCTION: Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation. {ECO:0000269|PubMed:11880369, ECO:0000269|PubMed:11956218, ECO:0000269|PubMed:12493770, ECO:0000269|PubMed:18024961}.

Molecular Weight: 74.9 kDa

UniProt: Q8TDF6

AlphaFold: Q8TDF6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling