KBTBD2 Protein (AA 1-623) (His tag)

Catalog No. ABIN7548683

This Recombinant KBTBD2 protein is expressed in HEK-293 Cells.

Quick Overview for KBTBD2 Protein (AA 1-623) (His tag) (ABIN7548683)

Target: KBTBD2 (Kelch Repeat and BTB (POZ) Domain Containing 2 (KBTBD2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-623

Purification tag / Conjugate: This KBTBD2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant KBTBD2 Protein expressed in mammalian cells.

Sequence: MSTQDERQIN TEYAVSLLEQ LKLFYEQQLF TDIVLIVEGT EFPCHKMVLA TCSSYFRAMF MSGLSESKQT HVHLRNVDAA TLQIIITYAY TGNLAMNDST VEQLYETACF LQVEDVLQRC REYLIKKINA ENCVRLLSFA DLFSCEELKQ SAKRMVEHKF TAVYHQDAFM QLSHDLLIDI LSSDNLNVEK EETVREAAML WLEYNTESRS QYLSSVLSQI RIDALSEVTQ RAWFQGLPPN DKSVVVQGLY KSMPKFFKPR LGMTKEEMMI FIEASSENPC SLYSSVCYSP QAEKVYKLCS PPADLHKVGT VVTPDNDIYI AGGQVPLKNT KTNHSKTSKL QTAFRTVNCF YWFDAQQNTW FPKTPMLFVR IKPSLVCCEG YIYAIGGDSV GGELNRRTVE RYDTEKDEWT MVSPLPCAWQ WSAAVVVHDC IYVMTLNLMY CYFPRSDSWV EMAMRQTSRS FASAAAFGDK IFYIGGLHIA TNSGIRLPSG TVDGSSVTVE IYDVNKNEWK MAANIPAKRY SDPCVRAVVI SNSLCVFMRE THLNERAKYV TYQYDLELDR WSLRQHISER VLWDLGRDFR CTVGKLYPSC LEESPWKPPT YLFSTDGTEE FELDGEMVAL PPV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: KBTBD2 (Kelch Repeat and BTB (POZ) Domain Containing 2 (KBTBD2))

Alternative Name: KBTBD2

Background: Kelch repeat and BTB domain-containing protein 2 (BTB and kelch domain-containing protein 1),FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of the insulin signaling pathway, modulating insulin sensitivity by limiting PIK3R1/p85alpha abundance in adipocytes. Targets PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase (PI3K), for 'Lys-48'-linked polyubiquitination and proteasome-mediated degradation. {ECO:0000269|PubMed:27708159}.

Molecular Weight: 71.3 kDa

UniProt: Q8IY47

AlphaFold: Q8IY47