MAGEF1 Protein (AA 1-307) (His tag)

Catalog No. ABIN7549228

This Recombinant MAGEF1 protein is produced in HEK-293 Cells.

Quick Overview for MAGEF1 Protein (AA 1-307) (His tag) (ABIN7549228)

Target: MAGEF1 (Melanoma Antigen Family F, 1 (MAGEF1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-307

Purification tag / Conjugate: This MAGEF1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant MAGEF1 Protein expressed in mammalian cells.

Sequence: MLQTPESRGL PVPQAEGEKD GGHDGETRAP TASQERPKEE LGAGREEGAA EPALTRKGAR ALAAKALARR RAYRRLNRTV AELVQFLLVK DKKKSPITRS EMVKYVIGDL KILFPDIIAR AAEHLRYVFG FELKQFDRKH HTYILINKLK PLEEEEEEDL GGDGPRLGLL MMILGLIYMR GNSAREAQVW EMLRRLGVQP SKYHFLFGYP KRLIMEDFVQ QRYLSYRRVP HTNPPEYEFS WGPRSNLEIS KMEVLGFVAK LHKKEPQHWP VQYREALADE ADRARAKARA EASMRARASA RAGIHLW Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MAGEF1 (Melanoma Antigen Family F, 1 (MAGEF1))

Alternative Name: MAGEF1

Background: Melanoma-associated antigen F1 (MAGE-F1) (MAGE-F1 antigen),FUNCTION: Enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin ligases. Proposed to act through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. MAGEF1-NSMCE1 ubiquitin ligase complex promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. May negatively regulate genome integrity by inhibiting homologous recombination-mediated double-strand break DNA repair (PubMed:29225034). {ECO:0000269|PubMed:20864041, ECO:0000269|PubMed:29225034}.

Molecular Weight: 35.2 kDa

UniProt: Q9HAY2

AlphaFold: Q9HAY2