MAFK Protein (AA 1-156) (His tag)
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- Target See all MAFK Proteins
- MAFK (Transcription Factor MafK (MAFK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-156
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MAFK protein is labelled with His tag.
- Purpose
- Made-to-order recombinant MAFK Protein expressed in mammalian cells.
- Sequence
- MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART VARGPVAPSK VATTSVITIV KSTELSSTSV PFSAAS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Transcription Factor MafK (MAFK) (AA 1-156) protein (His tag)
custom-made MAFK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Transcription Factor MafK (MAFK) (AA 1-110) protein (GST tag)MAFK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Transcription Factor MafK (MAFK) protein (His tag)MAFK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
Transcription Factor MafK (MAFK) proteinMAFK Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- MAFK (Transcription Factor MafK (MAFK))
- Alternative Name
- MAFK (MAFK Products)
- Background
- Transcription factor MafK (Erythroid transcription factor NF-E2 p18 subunit),FUNCTION: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:9150357). However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed:9150357, PubMed:8932385). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed:9150357). {ECO:0000269|PubMed:8932385, ECO:0000269|PubMed:9150357}.
- Molecular Weight
- 17.5 kDa
- UniProt
- O60675
- AlphaFold
- O60675
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