OTUD1 Protein (AA 1-481) (His tag)

Catalog No. ABIN7550332

This Recombinant OTUD1 protein is produced in HEK-293 Cells.

Quick Overview for OTUD1 Protein (AA 1-481) (His tag) (ABIN7550332)

Target: OTUD1 (OTU Domain Containing 1 (OTUD1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-481

Purification tag / Conjugate: This OTUD1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant OTUD1 Protein expressed in mammalian cells.

Sequence: MQLYSSVCTH YPAGAPGPTA AAPAPPAAAT PFKVSLQPPG AAGAAPEPET GECQPAAAAE HREAAAVPAA KMPAFSSCFE VVSGAAAPAS AAAGPPGASC KPPLPPHYTS TAQITVRALG ADRLLLHGPD PVPGAAGSAA APRGRCLLLA PAPAAPVPPR RGSSAWLLEE LLRPDCPEPA GLDATREGPD RNFRLSEHRQ ALAAAKHRGP AATPGSPDPG PGPWGEEHLA ERGPRGWERG GDRCDAPGGD AARRPDPEAE APPAGSIEAA PSSAAEPVIV SRSDPRDEKL ALYLAEVEKQ DKYLRQRNKY RFHIIPDGNC LYRAVSKTVY GDQSLHRELR EQTVHYIADH LDHFSPLIEG DVGEFIIAAA QDGAWAGYPE LLAMGQMLNV NIHLTTGGRL ESPTVSTMIH YLGPEDSLRP SIWLSWLSNG HYDAVFDHSY PNPEYDNWCK QTQVQRKRDE ELAKSMAISL SKMYIEQNAC S Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: OTUD1 (OTU Domain Containing 1 (OTUD1))

Alternative Name: OTUD1

Background: OTU domain-containing protein 1 (EC 3.4.19.12) (DUBA-7),FUNCTION: Deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PubMed:23827681). Required for the stability and translation of a subset mRNAs with a high abundance of rare codons by mediating deubiquitination of 40S ribosomal protein RPS10/eS10, thereby antagonizing ZNF598-mediated 40S ubiquitination (PubMed:36445135). The abundance of rare codons in mRNAs can limit the translation rate and can lead to ribosome collisions that trigger activation of ribosome quality control (RQC) pathway by ZNF598 (PubMed:36445135). OTUD1-mediated deubiquitination prevents activation of the RQC and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PubMed:36445135). {ECO:0000269|PubMed:23827681, ECO:0000269|PubMed:36445135}.

Molecular Weight: 51.1 kDa

UniProt: Q5VV17

AlphaFold: Q5VV17