KIAA0101 Protein (AA 1-111) (His tag)

Catalog No. ABIN7550595

Product Details

Target: KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))

Protein Type: Recombinant

Protein Characteristics: AA 1-111

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This KIAA0101 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant PCLAF Protein expressed in mammalian cells.

Sequence: MVRTKADSVP GTYRKVVAAR APRKVLGSST SATNSTSVSS RKAENKYAGG NPVCVRPTPK WQKGIGEFFR LSPKDSEKEN QIPEEAGSSG LGKAKRKACP LQPDHTNDEK E Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))

Alternative Name: PCLAF (PAF Products)

Background: PCNA-associated factor (Hepatitis C virus NS5A-transactivated protein 9) (HCV NS5A-transactivated protein 9) (Overexpressed in anaplastic thyroid carcinoma 1) (OEATC-1) (PCNA-associated factor of 15 kDa) (PAF15) (p15PAF) (PCNA-clamp-associated factor),FUNCTION: PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number. {ECO:0000269|PubMed:21673012, ECO:0000269|PubMed:23000965}.

Molecular Weight: 12.0 kDa

UniProt: Q15004

AlphaFold: Q15004

Pathways: Chromatin Binding