PTCHD4 Protein (AA 1-846) (His tag)

Catalog No. ABIN7551082

This Recombinant PTCHD4 protein is produced in HEK-293 Cells.

Quick Overview for PTCHD4 Protein (AA 1-846) (His tag) (ABIN7551082)

Target: PTCHD4 (Patched Domain Containing 4 (PTCHD4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-846

Purification tag / Conjugate: This PTCHD4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant PTCHD4 Protein expressed in mammalian cells.

Sequence: MCFLRRPGAP ASWIWWRMLR QVLRRGLQSF CHRLGLCVSR HPVFFLTVPA VLTITFGLSA LNRFQPEGDL ERLVAPSHSL AKIERSLASS LFPLDQSKSQ LYSDLHTPGR YGRVILLSPT GDNILLQAEG ILQTHRAVLE MKDGRNSFIG HQLGGVVEVP NSKDQRVKSA RAIQITYYLQ TYGSATQDLI GEKWENEFCK LIRKLQEEHQ ELQLYSLASF SLWRDFHKTS ILARSKVLVS LVLILTTATL SSSMKDCLRS KPFLGLLGVL TVCISIITAA GIFFITDGKY NSTLLGIPFF AMGHGTKGVF ELLSGWRRTK ENLPFKDRIA DAYSDVMVTY TMTSSLYFIT FGMGASPFTN IEAVKVFCQN MCVSILLNYF YIFSFFGSCL VFAGQLEQNR YHSIFCCKIP SAEYLDRKPV WFQTVMSDGH QQTSHHETNP YQHHFIQHFL REHYNEWITN IYVKPFVVIL YLIYASFSFM GCLQISDGAN IINLLASDSP SVSYAMVQQK YFSNYSPVIG FYVYEPLEYW NSSVQDDLRR LCSGFTAVSW VEQYYQFLKV SNVSANNKSD FISVLQSSFL KKPEFQHFRN DIIFSKAGDE SNIIASRLYL VARTSRDKQK EITEVLEKLR PLSLSKSIRF IVFNPSFVFM DHYSLSVTVP VLIAGFGVLL VLILTFFLVI HPLGNFWLIL SVTSIELGVL GLMTLWNVDM DCISILCLIY TLNFAIDHCA PLLFTFVLAT EHTRTQCIKS SLQDHGTAIL QNVTSFLIGL VPLLFVPSNL TFTLFKCLLL TGGCTLLHCF VILPVFLTFF PPSKKHHKKK KRAKRKEREE IECIEIQENP DHVTTV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PTCHD4 (Patched Domain Containing 4 (PTCHD4))

Alternative Name: PTCHD4

Background: Patched domain-containing protein 4 (p53-regulated patched protein),FUNCTION: Could act as a repressor of canonical hedgehog signaling by antagonizing the effects of SMO, as suggested by down-regulation of hedgehog target genes, including GLI1, PTCH1, and PTCH2 in PTCHD4-expressing cells. {ECO:0000269|PubMed:25296753}.

Molecular Weight: 96.4 kDa

UniProt: Q6ZW05

AlphaFold: Q6ZW05