Riboflavin Kinase Protein (RFK) (AA 1-155) (His tag)
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- Target See all Riboflavin Kinase (RFK) Proteins
- Riboflavin Kinase (RFK)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-155
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Riboflavin Kinase protein is labelled with His tag.
- Purpose
- Made-to-order recombinant RFK Protein expressed in mammalian cells.
- Sequence
- MRHLPYFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DISTGIYYGW ASVGSGDVHK MVVSIGWNPY YKNTKKSMET HIMHTFKEDF YGEILNVAIV GYLRPEKNFD SLESLISAIQ GDIEEAKKRL ELPEHLKIKE DNFFQVSKSK IMNGH Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Riboflavin Kinase (RFK) (AA 1-155) protein (His tag)
custom-made RFK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Riboflavin Kinase (RFK) (AA 1-162) protein (GST tag)RFK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Riboflavin Kinase (RFK) protein (His tag)RFK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % as determined by reducing SDS-PAGE.
Riboflavin Kinase (RFK) (AA 1-162) protein (His tag)RFK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Riboflavin Kinase (RFK) protein (Myc-DYKDDDDK Tag)RFK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Riboflavin Kinase (RFK)
- Alternative Name
- RFK (RFK Products)
- Background
- Riboflavin kinase (EC 2.7.1.26) (ATP:riboflavin 5'-phosphotransferase) (Flavokinase),FUNCTION: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. {ECO:0000269|PubMed:19641494}.
- Molecular Weight
- 17.6 kDa
- UniProt
- Q969G6
- AlphaFold
- Q969G6
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