RUFY4 Protein (AA 1-571) (His tag)

Catalog No. ABIN7551673

This Recombinant RUFY4 protein is produced in HEK-293 Cells.

Quick Overview for RUFY4 Protein (AA 1-571) (His tag) (ABIN7551673)

Target: RUFY4 (RUN and FYVE Domain Containing 4 (RUFY4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-571

Purification tag / Conjugate: This RUFY4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant RUFY4 Protein expressed in mammalian cells.

Sequence: MAEEGAILKV TKDLRAAVSA ILQGYGDGQG PVTDTSAELH RLCGCLELLL QFDQKEQKSF LGPRKDYWDF LCTALRRQRG NMEPIHFVRS QDKLKTPLGK GRAFIRFCLA RGQLAEALQL CLLNSELTRE WYGPRSPLLC PERQEDILDS LYALNGVAFE LDLQQPDLDG AWPMFSESRC SSSTQTQGRR PRKNKDAPKK IPAAYGGPEN VQIEDSHTSQ AICLQDAPSG QQLAGLPRSQ QQRHLPFFLE KKGESSRKHR YPQSMWEPEG KELQLDQEER APWIEIFLGN STPSTQGQGK GAMGTQKEVI GMEAEVTGVL LVAEGQRTTE GTHKKEAEWS HVQRLLMPSP RGAVEGAVSG SRQGSGGSSI LGEPWVLQGH ATKEDSTVEN PQVQTEVTLV ARREEQAEVS LQDEIKSLRL GLRKAEEQAQ RQEQLLREQE GELQALREQL SRCQEERAEL QAQLEQKQQE AERRDAMYQE ELGGQRDLVQ AMKRRVLELI QEKDRLWQRL QHLSSMAPEC CVACSKIFGR FSRRYPCRLC GGLLCHACSM DYKKRDRCCP PCAQGREAQV T Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RUFY4 (RUN and FYVE Domain Containing 4 (RUFY4))

Alternative Name: RUFY4

Background: RUN and FYVE domain-containing protein 4,FUNCTION: Positively regulates macroautophagy in primary dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger. {ECO:0000269|PubMed:26416964}.

Molecular Weight: 64.4 kDa

UniProt: Q6ZNE9

AlphaFold: Q6ZNE9