ARHGEF4 Protein (AA 1-690) (His tag)

Catalog No. ABIN7552204

This Recombinant ARHGEF4 protein is produced in HEK-293 Cells.

Quick Overview for ARHGEF4 Protein (AA 1-690) (His tag) (ABIN7552204)

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-690

Purification tag / Conjugate: This ARHGEF4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant ARHGEF4 Protein expressed in mammalian cells.

Sequence: MPWEEPAGEK PSCSHSQKAF HMEPAQKPCF TTDMVTWALL CISAETVRGE APSQPRGIPH RSPVSVDDLW LEKTQRKKLQ KQAHVERRLH IGAVHKDGVK CWRKTIITSP ESLNLPRRSH PLSQSAPTGL NHMGWPEHTP GTAMPDGALD TAVCADEVGS EEDLYDDLHS SSHHYSHPGG GGEQLAINEL ISDGSVVCAE ALWDHVTMDD QELGFKAGDV IEVMDATNRE WWWGRVADGE GWFPASFVRL RVNQDEPADD DAPLAGNSGA EDGGAEAQSS KDQMRTNVIN EILSTERDYI KHLRDICEGY VRQCRKRADM FSEEQLRTIF GNIEDIYRCQ KAFVKALEQR FNRERPHLSE LGACFLEHQA DFQIYSEYCN NHPNACVELS RLTKLSKYVY FFEACRLLQK MIDISLDGFL LTPVQKICKY PLQLAELLKY THPQHRDFKD VEAALHAMKN VAQLINERKR RLENIDKIAQ WQSSIEDWEG EDLLVRSSEL IYSGELTRVT QPQAKSQQRM FFLFDHQLIY CKKDLLRRDV LYYKGRLDMD GLEVVDLEDG KDRDLHVSIK NAFRLHRGAT GDSHLLCTRK PEQKQRWLKA FAREREQVQL DQETGFSITE LQRKQAMLNA SKQQVTGKPK AVGRPCYLTR QKHPALPSNR PQQQVLVLAE PRRKPSTFWH SISRLAPFRK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Alternative Name: ARHGEF4

Background: Rho guanine nucleotide exchange factor 4 (APC-stimulated guanine nucleotide exchange factor 1) (Asef) (Asef1),FUNCTION: Acts as a guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. {ECO:0000269|PubMed:10947987, ECO:0000269|PubMed:12598901, ECO:0000269|PubMed:17145773, ECO:0000269|PubMed:17599059, ECO:0000269|PubMed:19893577}.

Molecular Weight: 79.1 kDa

UniProt: Q9NR80

AlphaFold: Q9NR80

Pathways: Neurotrophin Signaling Pathway