BID Protein (AA 1-195) (His tag)

Catalog No. ABIN7552807

Product Details

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Protein Type: Recombinant

Protein Characteristics: AA 1-195

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This BID protein is labelled with His tag.

Purpose: Predefined custom protein recombinant BID Protein expressed in mammalian cells.

Sequence: MDCEVNNGSS LRDECITNLL VFGFLQSCSD NSFRRELDAL GHELPVLAPQ WEGYDELQTD GNRSSHSRLG RIEADSESQE DIIRNIARHL AQVGDSMDRS IPPGLVNGLA LQLRNTSRSE EDRNRDLATA LEQLLQAYPR DMEKEKTMLV LALLLAKKVA SHTPSLLRDV FHTTVNFINQ NLRTYVRSLA RNGMD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID (BID Products)

Background: BH3-interacting domain death agonist (p22 BID) (BID) [Cleaved into: BH3-interacting domain death agonist p15 (p15 BID), BH3-interacting domain death agonist p13 (p13 BID), BH3-interacting domain death agonist p11 (p11 BID)],FUNCTION: Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity). {ECO:0000250|UniProtKB:P70444, ECO:0000269|PubMed:14583606}., FUNCTION: [BH3-interacting domain death agonist p15]: Induces caspase activation and apoptosis (PubMed:15661737, PubMed:32029622). Allows the release of cytochrome c (PubMed:32029622). {ECO:0000269|PubMed:15661737, ECO:0000269|PubMed:32029622}., FUNCTION: [Isoform 1]: Induces ICE-like proteases and apoptosis. {ECO:0000269|PubMed:14583606}., FUNCTION: [Isoform 2]: Induces ICE-like proteases and apoptosis. {ECO:0000269|PubMed:14583606}., FUNCTION: [Isoform 3]: Does not induce apoptosis. {ECO:0000269|PubMed:14583606}., FUNCTION: [Isoform 4]: Induces ICE-like proteases and apoptosis. {ECO:0000269|PubMed:14583606}.

Molecular Weight: 22.0 kDa

UniProt: P55957

AlphaFold: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity