CCDC25 Protein (AA 1-208) (His tag)

Catalog No. ABIN7553010

Recombinant CCDC25 protein expressed in HEK-293 Cells.

Quick Overview for CCDC25 Protein (AA 1-208) (His tag) (ABIN7553010)

Target: CCDC25 (Coiled-Coil Domain Containing 25 (CCDC25))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-208

Purification tag / Conjugate: This CCDC25 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant CCDC25 Protein expressed in mammalian cells.

Sequence: MVFYFTSSSV NSSAYTIYMG KDKYENEDLI KHGWPEDIWF HVDKLSSAHV YLRLHKGENI EDIPKEVLMD CAHLVKANSI QGCKMNNVNV VYTPWSNLKK TADMDVGQIG FHRQKDVKIV TVEKKVNEIL NRLEKTKVER FPDLAAEKEC RDREERNEKK AQIQEMKKRE KEEMKKKREM DELRSYSSLM KVENMSSNQD GNDSDEFM Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CCDC25 (Coiled-Coil Domain Containing 25 (CCDC25))

Alternative Name: CCDC25

Background: Coiled-coil domain-containing protein 25,FUNCTION: Transmembrane receptor that senses neutrophil extracellular traps (NETs) and triggers the ILK-PARVB pathway to enhance cell motility (PubMed:32528174). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:32528174). Formation of NETs is also associated with cancer metastasis, NET-DNA acting as a chemotactic factor to attract cancer cells (PubMed:32528174). Specifically binds NETs on its extracellular region, in particular the 8-OHdG-enriched DNA present in NETs, and recruits ILK, initiating the ILK-PARVB cascade to induce cytoskeleton rearrangement and directional migration of cells (PubMed:32528174). In the context of cancer, promotes cancer metastasis by sensing NETs and promoting migration of tumor cells (PubMed:32528174). {ECO:0000269|PubMed:32528174}.

Molecular Weight: 24.5 kDa

UniProt: Q86WR0

AlphaFold: Q86WR0