DHX8 Protein (AA 1-1220) (His tag)

Catalog No. ABIN7553665

Product Details

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Protein Type: Recombinant

Protein Characteristics: AA 1-1220

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This DHX8 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant DHX8 Protein expressed in mammalian cells.

Sequence: MAVAVAMAGA LIGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLIQ TMRPPAKPST SKDPVVKPKT EKEKLKELFP VLCQPDNPSV RTMLDEDDVK VAVDVLKELE ALMPSAAGQE KQRDAEHRDR TKKKKRSRSR DRNRDRDRDR ERNRDRDHKR RHRSRSRSRS RTRERNKVKS RYRSRSRSQS PPKDRKDRDK YGERNLDRWR DKHVDRPPPE EPTIGDIYNG KVTSIMQFGC FVQLEGLRKR WEGLVHISEL RREGRVANVA DVVSKGQRVK VKVLSFTGTK TSLSMKDVDQ ETGEDLNPNR RRNLVGETNE ETSMRNPDRP THLSLVSAPE VEDDSLERKR LTRISDPEKW EIKQMIAANV LSKEEFPDFD EETGILPKVD DEEDEDLEIE LVEEEPPFLR GHTKQSMDMS PIKIVKNPDG SLSQAAMMQS ALAKERRELK QAQREAEMDS IPMGLNKHWV DPLPDAEGRQ IAANMRGIGM MPNDIPEWKK HAFGGNKASY GKKTQMSILE QRESLPIYKL KEQLVQAVHD NQILIVIGET GSGKTTQITQ YLAEAGYTSR GKIGCTQPRR VAAMSVAKRV SEEFGCCLGQ EVGYTIRFED CTSPETVIKY MTDGMLLREC LIDPDLTQYA IIMLDEAHER TIHTDVLFGL LKKTVQKRQD MKLIVTSATL DAVKFSQYFY EAPIFTIPGR TYPVEILYTK EPETDYLDAS LITVMQIHLT EPPGDILVFL TGQEEIDTAC EILYERMKSL GPDVPELIIL PVYSALPSEM QTRIFDPAPP GSRKVVIATN IAETSLTIDG IYYVVDPGFV KQKVYNSKTG IDQLVVTPIS QAQAKQRAGR AGRTGPGKCY RLYTERAYRD EMLTTNVPEI QRTNLASTVL SLKAMGINDL LSFDFMDAPP METLITAMEQ LYTLGALDDE GLLTRLGRRM AEFPLEPMLC KMLIMSVHLG CSEEMLTIVS MLSVQNVFYR PKDKQALADQ KKAKFHQTEG DHLTLLAVYN SWKNNKFSNP WCYENFIQAR SLRRAQDIRK QMLGIMDRHK LDVVSCGKST VRVQKAICSG FFRNAAKKDP QEGYRTLIDQ QVVYIHPSSA LFNRQPEWVV YHELVLTTKE YMREVTTIDP RWLVEFAPAF FKVSDPTKLS KQKKQQRLEP LYNRYEEPNA WRISRAFRRR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Alternative Name: DHX8 (DHX8 Products)

Background: ATP-dependent RNA helicase DHX8 (EC 3.6.4.13) (DEAH box protein 8) (RNA helicase HRH1),FUNCTION: Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28502770, PubMed:28076346). Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome (PubMed:8608946). {ECO:0000269|PubMed:11991638, ECO:0000269|PubMed:28076346, ECO:0000269|PubMed:28502770, ECO:0000269|PubMed:8608946}.

Molecular Weight: 139.3 kDa

UniProt: Q14562

AlphaFold: Q14562