FBXW10 Protein (AA 1-1052) (His tag)

Catalog No. ABIN7553874

Recombinant FBXW10 protein expressed in HEK-293 Cells.

Quick Overview for FBXW10 Protein (AA 1-1052) (His tag) (ABIN7553874)

Target: FBXW10 (F-Box and WD Repeat Domain Containing 10 (FBXW10))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1052

Purification tag / Conjugate: This FBXW10 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant FBXW10 Protein expressed in mammalian cells.

Sequence: MENLESRLKN APYFRCEKGT DSIPLCRKCE TCVLAWKIFS TKEWFCRIND ISQRRFLVGI LKQLNSLYLL HYFQNILQTT QGKDFIYNRS RINLSKKEGK VVKSSLNQML DKTVEQKMKE ILYWFANSTQ WTKANYTLLL LQMCNPKLLL TAANVIRVLF LREENNISGL NQDITDVCFS PEKDHSSKSA TSQVYWTAKT QHTSLPLSKA PENEHLLGAA SNPEEPWRNS LRCISEMNRL FSGKGDITKP GYDPCNLLVD LDDIRDLSSG FSKYRDFIRY LPIHLSKYIL RMLDRHTLNK CASVSQHWAA MAQQVKMDLS AHGFIQNQIT FLQGSYTRGI DPNYANKVSI PVPKMVDDGK SMRVKHPKWK LRTKNEYNLW TAYQNEETQQ VLIEERNVFC GTYNVRILSD TWDQNRVIHY SGGDLIAVSS NRKIHLLDII QVKAIPVEFR GHAGSVRALF LCEEENFLLS GSYDLSIRYW DLKSGVCTRI FGGHQGTITC MDLCKNRLVS GGRDCQVKVW DVDTGKCLKT FRHKDPILAT RINDTYIVSS CERGLVKVWH IAMAQLVKTL SGHEGAVKCL FFDQWHLLSG STDGLVMAWS MVGKYERCLM AFKHPKEVLD VSLLFLRVIS ACADGKIRIY NFLNGNCMKV LKANGRGDPV LSFFIQGNRM VVNTESNVLM FQFEHIKWQY AVEKTKQKKN KEKEEEKEEN SLMEILSKCN IQVHSPRESV SSKQTVIQEL LPGKPPKSRV LLKPAKFSSA VLIEELQSQG KSKSPRRDAD DVEKAQKQGQ LETPGKLPSH PKKKSWKIPM SPDQFLLTVS ALQHAHNSGE FAYPCRPQTE ITDVWGPSIS YPRKVLNFKG KSIQRAVDRL RLSNPPIDVK RTSIPLEIQK LQPNLKISLH SPRVQSTIPQ PMIIRSRFSG SLKGGDQVTS SIERAVCSTG PLTSMQVIKP NRMLAPQVGT ATLSLKKERP RIYTALDPFR VNTEFVLLTV KEEKEHQEAK MKEYQAREST GVVDPGKVSK AAWIRKIKGL PIDNFTKQGK TAAPELGQNV FI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FBXW10 (F-Box and WD Repeat Domain Containing 10 (FBXW10))

Alternative Name: FBXW10

Background: F-box/WD repeat-containing protein 10 (F-box and WD-40 domain-containing protein 10) (Ubiquitin ligase-specificity factor),FUNCTION: Probable substrate-recognition component of a SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Overexpression is leading to degradation of CBX5 and CBX1. {ECO:0000269|PubMed:20498703}.

Molecular Weight: 119.8 kDa

UniProt: Q5XX13

AlphaFold: Q5XX13