MAGEE1 Protein (AA 1-957) (His tag)

Catalog No. ABIN7554523

Product Details

Target: MAGEE1 (Melanoma Antigen Family E, 1 (MAGEE1))

Protein Type: Recombinant

Protein Characteristics: AA 1-957

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This MAGEE1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant MAGEE1 Protein expressed in mammalian cells.

Sequence: MSLVSQNSRR RRRRVAKATA HNSSWGEMQA PNAPGLPADV PGSDVPQGPS DSQILQGLCA SEGPSTSVLP TSAEGPSTFV PPTISEASSA SGQPTISEGP GTSVLPTPSE GLSTSGPPTI SKGLCTSVTL AASEGRNTSR PPTSSEEPST SVPPTASEVP STSLPPTPGE GTSTSVPPTA YEGPSTSVVP TPDEGPSTSV LPTPGEGPGT SVPLAATEGL STSVQATPDE GPSTSVPPTA TEGLSTPVPP TRDEGPSTSV PATPGEGPST SVLPAASDGQ SISLVPTRGK GSSTSVPPTA TEGLSTSVQP TAGEGSSTSV PPTPGGGLST SVPPTATEEL STSVPPTPGE GPSTSVLPIP GEGLSTSVPP TASDGSDTSV PPTPGEGAST LVQPTAPDGP GSSVLPNPGE GPSTLFSSSA SVDRNPSKCS LVLPSPRVTK ASVDSDSEGP KGAEGPIEFE VLRDCESPNS ISIMGLNTSR VAITLKPQDP MEQNVAELLQ FLLVKDQSKY PIRESEMREY IVKEYRNQFP EILRRAAAHL ECIFRFELRE LDPEAHTYIL LNKLGPVPFE GLEESPNGPK MGLLMMILGQ IFLNGNQAKE AEIWEMLWRM GVQRERRLSI FGNPKRLLSV EFVWQRYLDY RPVTDCKPVE YEFFWGPRSH LETTKMKILK FMAKIYNKDP MDWPEKYNEA LEEDAARAFA EGWQALPHFR RPFFEEAAAE VPSPDSEVSS YSSKYAPHSW PESRLESKAR KLVQLFLLMD STKLPIPKKG ILYYIGRECS KVFPDLLNRA ARTLNHVYGT ELVVLDPRNH SYTLYNRREM EETEEIVDSP NRPGNNFLMQ VLSFIFIMGN HARESAVWAF LRGLGVQAGR KHVITCRYLS QRYIDSLRVP DSDPVQYEFV WGPRARLETS KMKALRYVAR IHRKEPQDWP QQYREAMEDE ANRADVGHRQ IFVHNFR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MAGEE1 (Melanoma Antigen Family E, 1 (MAGEE1))

Alternative Name: MAGEE1 (MAGEE1 Products)

Background: Melanoma-associated antigen E1 (Alpha-dystrobrevin-associated MAGE Protein) (DAMAGE) (Hepatocellular carcinoma-associated protein 1) (MAGE-E1 antigen),FUNCTION: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. {ECO:0000269|PubMed:20864041}.

Molecular Weight: 103.3 kDa

UniProt: Q9HCI5

AlphaFold: Q9HCI5

Pathways: Synaptic Membrane