MED23 Protein (AA 1-1368) (His tag)
custom-made
MED23
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all MED23 Proteins
- MED23 (Mediator Complex Subunit 23 (MED23))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1368
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MED23 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant MED23 Protein expressed in mammalian cells.
- Sequence
- METQLQSIFE EVVKTEVIEE AFPGMFMDTP EDEKTKLISC LGAFRQFWGG LSQESHEQCI QWIVKFIHGQ HSPKRISFLY DCLAMAVETG LLPPRLVCES LINSDTLEWE RTQLWALTFK LVRKIIGGVD YKGVRDLLKV ILEKILTIPN TVSSAVVQQL LAAREVIAYI LERNACLLPA YFAVTEIRKL YPEGKLPHWL LGNLVSDFVD TFRPTARINS ICGRCSLLPV VNNSGAICNS WKLDPATLRF PLKGLLPYDK DLFEPQTALL RYVLEQPYSR DMVCNMLGLN KQHKQRCPVL EDQLVDLVVY AMERSETEEK FDDGGTSQLL WQHLSSQLIF FVLFQFASFP HMVLSLHQKL AGRGLIKGRD HLMWVLLQFI SGSIQKNALA DFLPVMKLFD LLYPEKEYIP VPDINKPQST HAFAMTCIWI HLNRKAQNDN SKLQIPIPHS LRLHHEFLQQ SLRNKSLQMN DYKIALLCNA YSTNSECFTL PMGALVETIY GNGIMRIPLP GTNCMASGSI TPLPMNLLDS LTVHAKMSLI HSIATRVIKL AHAKSSVALA PALVETYSRL LVYMEIESLG IKGFISQLLP TVFKSHAWGI LHTLLEMFSY RMHHIQPHYR VQLLSHLHTL AAVAQTNQNQ LHLCVESTAL RLITALGSSE VQPQFTRFLS DPKTVLSAES EELNRALILT LARATHVTDF FTGSDSIQGT WCKDILQTIM SFTPHNWASH TLSCFPGPLQ AFFKQNNVPQ ESRFNLKKNV EEEYRKWKSM SNENDIITHF SMQGSPPLFL CLLWKMLLET DHINQIGYRV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLLL LKPNDFRNRV SDFVKENSPE HWLQNDWHTK HMNYHKKYPE KLYFEGLAEQ VDPPVQIQSP YLPIYFGNVC LRFLPVFDIV IHRFLELLPV SKSLETLLDH LGGLYKFHDR PVTYLYNTLH YYEMHLRDRA FLKRKLVHAI IGSLKDNRPQ GWCLSDTYLK CAMNAREENP WVPDDTYYCR LIGRLVDTMA GKSPGPFPNC DWRFNEFPNP AAHALHVTCV ELMALAVSGK EVGNALLNVV LKSQPLVPRE NITAWMNAIG LIITALPEPY WIVLHDRIVS VISSPSLTSE TEWVGYPFRL FDFTACHQSY SEMSCSYTLA LAHAVWHHSS IGQLSLIPKF LTEVLLPIVK TEFQLLYVYH LVGPFLQRFQ QERTRCMIEI GVAFYDMLLN VDQCSTHLNY MDPICDFLYH MKYMFTGDSV KEQVEKIICN LKPALKLRLR FITHISKMEP AAVPPQAMNS GSPAPQSNQV PVSLPVTQ Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Mediator Complex Subunit 23 (MED23) (AA 1-1367) protein (His tag)
custom-made MED23 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- MED23 (Mediator Complex Subunit 23 (MED23))
- Alternative Name
- MED23 (MED23 Products)
- Background
- Mediator of RNA polymerase II transcription subunit 23 (Activator-recruited cofactor 130 kDa component) (ARC130) (Cofactor required for Sp1 transcriptional activation subunit 3) (CRSP complex subunit 3) (Mediator complex subunit 23) (Protein sur-2 homolog) (hSur-2) (Transcriptional coactivator CRSP130) (Vitamin D3 receptor-interacting protein complex 130 kDa component) (DRIP130),FUNCTION: Required for transcriptional activation subsequent to the assembly of the pre-initiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling. {ECO:0000250, ECO:0000269|PubMed:10353252, ECO:0000269|PubMed:14759369, ECO:0000269|PubMed:16595664}.
- Molecular Weight
- 156.5 kDa
- UniProt
- Q9ULK4
- AlphaFold
- Q9ULK4
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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