MCM10 Protein (AA 1-875) (His tag)

Catalog No. ABIN7554537

Recombinant MCM10 protein expressed in HEK-293 Cells.

Quick Overview for MCM10 Protein (AA 1-875) (His tag) (ABIN7554537)

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-875

Purification tag / Conjugate: This MCM10 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant MCM10 Protein expressed in mammalian cells.

Sequence: MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG DGESYTEEAD DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV LPAPAPRREK TNEELQEELR NLQEQMKALQ EQLKVTTIKQ TASPARLQKS PVEKSPRPPL KERRVQRIQE STCFSAELDV PALPRTKRVA RTPKASPPDP KSSSSRMTSA PSQPLQTISR NKPSGITRGQ IVGTPGSSGE TTQPICVEAF SGLRLRRPRV SSTEMNKKMT GRKLIRLSQI KEKMAREKLE EIDWVTFGVI LKKVTPQSVN SGKTFSIWKL NDLRDLTQCV SLFLFGEVHK ALWKTEQGTV VGILNANPMK PKDGSEEVCL SIDHPQKVLI MGEALDLGTC KAKKKNGEPC TQTVNLRDCE YCQYHVQAQY KKLSAKRADL QSTFSGGRIP KKFARRGTSL KERLCQDGFY YGGVSSASYA ASIAAAVAPK KKIQTTLSNL VVKGTNLIIQ ETRQKLGIPQ KSLSCSEEFK ELMDLPTCGA RNLKQHLAKA TASGIMGSPK PAIKSISASA LLKQQKQRML EMRRRKSEEI QKRFLQSSSE VESPAVPSSS RQPPAQPPRT GSEFPRLEGA PATMTPKLGR GVLEGDDVLF YDESPPPRPK LSALAEAKKL AAITKLRAKG QVLTKTNPNS IKKKQKDPQD ILEVKERVEK NTMFSSQAED ELEPARKKRR EQLAYLESEE FQKILKAKSK HTGILKEAEA EMQERYFEPL VKKEQMEEKM RNIREVKCRV VTCKTCAYTH FKLLETCVSE QHEYHWHDGV KRFFKCPCGN RSISLDRLPN KHCSNCGLYK WERDGMLKEK TGPKIGGETL LPRGEEHAKF LNSLK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Alternative Name: MCM10

Background: Protein MCM10 homolog (HsMCM10),FUNCTION: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability, acts as a direct target of transcriptional repression by ZBTB38 (PubMed:24726359). {ECO:0000269|PubMed:11095689, ECO:0000269|PubMed:15136575, ECO:0000269|PubMed:17699597, ECO:0000269|PubMed:19608746, ECO:0000269|PubMed:24726359, ECO:0000269|PubMed:32865517}.

Molecular Weight: 98.2 kDa

UniProt: Q7L590

AlphaFold: Q7L590

Pathways: Mitotic G1-G1/S Phases, DNA Replication