Nestin Protein (NES) (AA 1-1621) (His tag)

Catalog No. ABIN7554705

Product Details

Target: Nestin (NES)

Protein Type: Recombinant

Protein Characteristics: AA 1-1621

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Nestin protein is labelled with His tag.

Purpose: Predefined custom protein recombinant NES Protein expressed in mammalian cells.

Sequence: MEGCMGEESF QMWELNRRLE AYLARVKALE EQNELLSAEL GGLRAQSADT SWRAHADDEL AALRALVDQR WREKHAAEVA RDNLAEELEG VAGRCQQLRL ARERTTEEVA RNRRAVEAEK CARAWLSSQV AELERELEAL RVAHEEERVG LNAQAACAPR CPAPPRGPPA PAPEVEELAR RLGEAWRGAV RGYQERVAHM ETSLGQARER LGRAVQGARE GRLELQQLQA ERGGLLERRA ALEQRLEGRW QERLRATEKF QLAVEALEQE KQGLQSQIAQ VLEGRQQLAH LKMSLSLEVA TYRTLLEAEN SRLQTPGGGS KTSLSFQDPK LELQFPRTPE GRRLGSLLPV LSPTSLPSPL PATLETPVPA FLKNQEFLQA RTPTLASTPI PPTPQAPSPA VDAEIRAQDA PLSLLQTQGG RKQAPEPLRA EARVAIPASV LPGPEEPGGQ RQEASTGQSP EDHASLAPPL SPDHSSLEAK DGESGGSRVF SICRGEGEGQ IWGLVEKETA IEGKVVSSLQ QEIWEEEDLN RKEIQDSQVP LEKETLKSLG EEIQESLKTL ENQSHETLER ENQECPRSLE EDLETLKSLE KENKELLKDV EVVRPLEKEA VGQLKPTGKE DTQTLQSLQK ENQELMKSLE GNLETFLFPG TENQELVSSL QENLESLTAL EKENQEPLRS PEVGDEEALR PLTKENQEPL RSLEDENKEA FRSLEKENQE PLKTLEEEDQ SIVRPLETEN HKSLRSLEEQ DQETLRTLEK ETQQRRRSLG EQDQMTLRPP EKVDLEPLKS LDQEIARPLE NENQEFLKSL KEESVEAVKS LETEILESLK SAGQENLETL KSPETQAPLW TPEEINQGAM NPLEKEIQEP LESVEVNQET FRLLEEENQE SLRSLGAWNL ENLRSPEEVD KESQRNLEEE ENLGKGEYQE SLRSLEEEGQ ELPQSADVQR WEDTVEKDQE LAQESPPGMA GVENEDEAEL NLREQDGFTG KEEVVEQGEL NATEEVWIPG EGHPESPEPK EQRGLVEGAS VKGGAEGLQD PEGQSQQVGA PGLQAPQGLP EAIEPLVEDD VAPGGDQASP EVMLGSEPAM GESAAGAEPG PGQGVGGLGD PGHLTREEVM EPPLEEESLE AKRVQGLEGP RKDLEEAGGL GTEFSELPGK SRDPWEPPRE GREESEAEAP RGAEEAFPAE TLGHTGSDAP SPWPLGSEEA EEDVPPVLVS PSPTYTPILE DAPGPQPQAE GSQEASWGVQ GRAEALGKVE SEQEELGSGE IPEGPQEEGE ESREESEEDE LGETLPDSTP LGFYLRSPTS PRWDPTGEQR PPPQGETGKE GWDPAVLASE GLEAPPSEKE EGEEGEEECG RDSDLSEEFE DLGTEAPFLP GVPGEVAEPL GQVPQLLLDP AAWDRDGESD GFADEEESGE EGEEDQEEGR EPGAGRWGPG SSVGSLQALS SSQRGEFLES DSVSVSVPWD DSLRGAVAGA PKTALETESQ DSAEPSGSEE ESDPVSLERE DKVPGPLEIP SGMEDAGPGA DIIGVNGQGP NLEGKSQHVN GGVMNGLEQS EEVGQGMPLV SEGDRGSPFQ EEEGSALKTS WAGAPVHLGQ GQFLKFTQRE GDRESWSSGE D Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Nestin (NES)

Alternative Name: NES (NES Products)

Background: Nestin,FUNCTION: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). {ECO:0000250}.

Molecular Weight: 177.4 kDa

UniProt: P48681

AlphaFold: P48681