NOX5 Protein (AA 1-765) (His tag)

Catalog No. ABIN7554783

Recombinant NOX5 protein expressed in HEK-293 Cells.

Quick Overview for NOX5 Protein (AA 1-765) (His tag) (ABIN7554783)

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-765

Purification tag / Conjugate: This NOX5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant NOX5 Protein expressed in mammalian cells.

Sequence: MNTSGDPAQT GPEGCRGTMS AEEDARWLRW VTQQFKTIAG EDGEISLQEF KAALHVKESF FAERFFALFD SDRSGTITLQ ELQEALTLLI HGSPMDKLKF LFQVYDIDVC ARQGASAGTE WGAGAGPHWA SSPLGTGSGS IDPDELRTVL QSCLRESAIS LPDEKLDQLT LALFESADAD GNGAITFEEL RDELQRFPGV MENLTISAAH WLTAPAPRPR PRRPRQLTRA YWHNHRSQLF CLATYAGLHV LLFGLAASAH RDLGASVMVA KGCGQCLNFD CSFIAVLMLR RCLTWLRATW LAQVLPLDQN IQFHQLMGYV VVGLSLVHTV AHTVNFVLQA QAEASPFQFW ELLLTTRPGI GWVHGSASPT GVALLLLLLL MFICSSSCIR RSGHFEVFYW THLSYLLVWL LLIFHGPNFW KWLLVPGILF FLEKAIGLAV SRMAAVCIME VNLLPSKVTH LLIKRPPFFH YRPGDYLYLN IPTIARYEWH PFTISSAPEQ KDTIWLHIRS QGQWTNRLYE SFKASDPLGR GSKRLSRSVT MRKSQRSSKG SEILLEKHKF CNIKCYIDGP YGTPTRRIFA SEHAVLIGAG IGITPFASIL QSIMYRHQKR KHTCPSCQHS WIEGVQDNMK LHKVDFIWIN RDQRSFEWFV SLLTKLEMDQ AEEAQYGRFL ELHMYMTSAL GKNDMKAIGL QMALDLLANK EKKDSITGLQ TRTQPGRPDW SKVFQKVAAE KKGKVQVFFC GSPALAKVLK GHCEKFGFRF FQENF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Alternative Name: NOX5

Background: NADPH oxidase 5 (EC 1.6.3.-),FUNCTION: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed:12686516). May play a role in cell growth and apoptosis (PubMed:12686516). {ECO:0000269|PubMed:12686516}., FUNCTION: [Isoform v2]: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed:11483596, PubMed:14982937, PubMed:17275676, PubMed:21642394, PubMed:24505490, PubMed:36653838, PubMed:17587483, PubMed:22427510, PubMed:22387196). Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa (PubMed:11483596). Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin (PubMed:17275676). {ECO:0000269|PubMed:11483596, ECO:0000269|PubMed:14982937, ECO:0000269|PubMed:17275676, ECO:0000269|PubMed:17587483, ECO:0000269|PubMed:21642394, ECO:0000269|PubMed:22387196, ECO:0000269|PubMed:22427510, ECO:0000269|PubMed:24505490, ECO:0000269|PubMed:36653838}., FUNCTION: [Isoform v1]: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. {ECO:0000269|PubMed:21319793, ECO:0000269|PubMed:22427510}., FUNCTION: [Isoform v5]: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed:17275676, PubMed:36653838). According to PubMed:22427510, lacks enzyme activity (PubMed:22427510). Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin (PubMed:17275676). {ECO:0000269|PubMed:17275676, ECO:0000269|PubMed:22427510, ECO:0000269|PubMed:36653838}., FUNCTION: [Isoform v4]: Lacks calcium-dependent NADPH oxidase activity. {ECO:0000269|PubMed:22427510}., FUNCTION: [Isoform v3]: Lacks calcium-dependent NADPH oxidase activity. {ECO:0000269|PubMed:22427510}.

Molecular Weight: 86.4 kDa

UniProt: Q96PH1

AlphaFold: Q96PH1

Pathways: Proton Transport